Filter paper was placed at the bottom of the extraction cell to prevent clogging of extraction tubing and ensure consistent extraction volumes. We describe a new technique for sample preparation, accelerated solvent extraction ase, that combines elevated temperatures and pressures with liquid solvents. Accelerated solvent extraction is an innovative sample preparation technique that combines elevated temperatures and pressures with liquid solvents to achieve fast and efficient removal of analytes of interest from various matrices. The dionex ase 300 has the capability to extract samples with volumes as large as 100 ml. Thermo scientific dionex ase 150350 stainless steel. The process uses high temperature and pressure, which results in the extraction taking less time and requiring less solvent, and possibly also giving better analyte recovery, than traditional methods that use less extreme conditions. Accelerated solvent extraction is a technique for extracting organic compounds from solid and semisolid samples with liquid solvents.
Thermo scientific dionex ase 150350 dionium extraction. Accelerated solvent extraction ondemand webinar and faq. Improved extractions with accelerated solvent extraction. Rapid determination of total fat from dairy products.
Since analytes diffuse out of the matrix and into the extraction solvent, static extraction cycles maximize the concentration gradient that permits this movement and results in. For a limited time, upgrade to a dionex ase 350 accelerated solvent extractor system. Need to replace the cylindrical body of the dionium extraction cell in your thermo scientific dionex ase 150 or 350 accelerated solvent extractor. The dionex ase 350 system can automatically extract up to 24 samples, using less solvent and labor, and accommodates 1, 5, 10, 22, 34, 66 and 100 ml cell sizes.
The original extraction procedure can be found in an 325. To further reduce solvent use, the dionex ase 350 supports a unique extraction method called the solvent saver mode. Pressurized liquid extraction was performed with an accelerated solvent extraction system ase 350, dionex, ca, usa. Using accelerated solvent extraction ase accelerated solvent extraction of unsaturated fats in popular snack foods introduction accelerated solvent extraction ase is an extraction method that significantly streamlines sample preparation. Dionex ase 350 accelerated solvent extractor system. Introduction to accelerated solvent extraction youtube. We carry extraction cell parts, peek washers, collection vials, orings, filters, tools, and more, all economicall\ y priced and designed to meet or exceed the performance of the original manufacturer s parts. Accelerated solvent extraction has been demonstrated to be equivalent to existing extraction methodologies such as soxhlet and automated soxhlet. Accelerated solvent extraction for additivies in polymer. Rapid determination of total fat from dairy products application note 364 introduction sample preparationspecifically, solvent extraction is an important step in analytical processes. Accelerated solvent extraction technique was demonstrated on a transdermal patch pouch and a polypropylene pill bottle using the automated dionex ase 350. The thermo scientific dionex ase 350 accelerated solvent extractor and dionex autotrace 280 solidphase extraction spe systems use less solvent and take less time and labor than other sample preparation technologies while delivering superior analytical performance.
Dionex ase systems use organic and aqueous liquid solvents at elevated temperatures and. There are limited studies on the effects of extraction conditions using the ase technique to achieve optimum oil yield. The accelerated solvent extraction method significantly streamlines sample preparation. Determination of persistent organic pollutants in fish tissue by accelerated solvent extraction and gcmsms the extractions were carried out using a thermo scientific dionex ase 350 accelerated solvent extractor figure 1 equipped with 34 ml stainless steel extraction cells. Thermo scientific dionex ase 350 accelerated solvent. Accelerated solvent extraction conditions oven temperature. A commonly used solvent is pumped into an extraction cell containing the sample, which is then. Accelerated solvent extraction technique provides automated and more efficient. Solvent extraction is typically employed for the extraction of oil. Comparison of soxhlet and accelerated solvent extraction for packing material leachable and extractable studies using lcms. Through use of the static extraction cycles, the accelerated solvent extraction technique assures an exhaustive extraction in a manner that uses much less solvent and time. Ase 200 accelerated solvent extractor operators manual.
Dionex ase 350 accelerated solvent extractor operators manual. Learn about the accelerated solvent extraction technique, one of the fastest, safest and easiest techniques available for extracting analytes out of solid and semisolid samples. Equipment dionex ase 350 accelerated solvent extractor system pn 083114. Extraction of pahs from environmental samples by accelerated solvent extraction ase meets the requirements of u. Each system includes one solvent bottle, snapring pliers, filter insertion tool, oring insertion tool, diatomaceous earth, power cords, and gas line fittings. Choose thermo scientific dionex ase 150300 extraction cell bodiesthe long cylindrical portion of the extraction cellif a replacement is needed for your thermo scientific dionex ase 150 and 350 accelerated solvent extractors. Samples, typically 6 g milled samples, were placed into stainless steel extraction chambers. Extractions were performed with the model 200 accelerated solvent extractor, dionex ase 350. Extraction of oil content from oilseeds by accelerated. Dionex ase 350 accelerated solvent extractor rocket evaporator system a cellulose filter was placed in the bottom of a 34 ml extraction cell figure 2, followed by 5 g of activated florisil and another cellulose filter. All samples were extracted by a dionex accelerated solvent extraction system ase350 equipped with stainless steel extraction cells 34 ml from thermo scientific waltham, ma, usa. Thermo scientific dionex ase 150 and ase 350 accelerated solvent extractor systems. Rapid determination of total fat from dairy products application note 364 introduction sample preparationspecifically, solvent extractionis an important step in analytical processes.
The thermo scientific dionex ase 150 350 accelerated solvent extraction system is designed to provide walkaway automation and can extract 124 samples in a single batch. Page 3 performing ase add solid or semisolid samples that have been chemically treated to the ase dionium extraction cell. Restek offers a wide range of replacement parts to keep your. The thermo scientific dionex ase 150350 accelerated solvent extraction system is designed to provide walkaway automation and can extract 124 samples in a single batch. Extraction of organochlorine pesticides from oyster tissue. What is the abbreviation for accelerated solvent extraction. A 2 g sample of honey was homogenized with an equal. Pdf accelerated solvent extraction for natural products. You will save time, solvent, and money, and generate results in a fraction of the time compared to traditional techniques such as soxhlet. Values are corrected for recovery of 5 brief of application note 323 extraction of polychlorinated dibenzop. The extraction pressure 1500 psi was imposed by the ase 350 apparatus. In the case of spiked samples the spikes were added to the sample prior to extraction. Ase 200 accelerated solvent extractor, with 11 or 22 ml stainless steel extraction cells cellulose filters pn 049458 collection vials, 40 ml pn.
Comparison of soxhlet and accelerated solvent extraction. The thermo scientific dionex ase 350 accelerated solvent extraction system uses a combination of elevated. The system uses conventional solvents at elevated temperatures and pressures, which results in improved extraction kinetics. Dionex ase 350 accelerated solvent extractor and rocket evaporator. Accelerated solvent extraction ase is a method for extracting various chemicals from a complex solid or semisolid sample matrix. Unsurpassed extraction technology accelerated solvent. For many years, analysts have used an array of solvent extraction techniques, such as soxhlet, automated soxhlet, or sonication. Check out theaccelerated solvent extraction brochure downloadable pdf. After heating, the extract is flushed from the sample cell into a standard collection vial and is ready for analysis. Accelerated solvent extraction applications series register to learn how you can use thermo scientific technology to test for methods for extracting fats and environmental contaminants from a variety of food and beverage samples. Accelerated solvent extraction technique provides automated and more efficient extractions than the traditional soxhlet extraction method and require less time and less solvent. Dionex ase 350 accelerated solvent extractor operators. Restek offers a wide range of replacement parts and accessories for accelerated solvent extraction to keep your ase system running smoothly.
This system accommodates sample sizes of 1100g, allows unattended extraction of up to 24 samples and uses 50 to 9. And other extractions were used by accelerated solvent extraction by ethanol. Advantages of using accelerated solvent extraction technique. Ultrapure water was obtained from a milliq water purification system integral 5 millipore, ma, usa. Dionex accelerated solvent extraction ase free download as powerpoint presentation. Available in 66ml and 100ml, these dionex ase 150350 dionium extraction cell bodies are resistant to low concentrations of mineral acids and strong bases. The topic of the webinar was on the technique of accelerated solvent extraction ase.
Automate extraction, filtration and clean up of compounds from solid and semisolid samples in minutes using the automated thermo scientific dionex ase 350 accelerated solvent extractor. Accelerated solvent extraction is a proven technique that speeds up the gravimetric fat determination in food products such as chocolate and greatly reduces the amount of solvent used. Gravimetric analysis were performed on a scale with d 0. Accelerated solvent extraction ase, first introduced in 1995, is an automated rapid extraction technique that utilizes common solvents at elevated temperature and pressure, and thereby.
Accelerated solvent extraction for alternative fuel research. Automatically extract up to 24 samples 1100 grams each. Last year, our salt lake center of technical excellence experts presented a very popular webinar, titled, innovations in automated sample preparation 52 min. Pressure is applied to the sample extraction cell to maintain the heated solvent in a liquid state during the extraction. Thermo scientific dionex ase 150 and ase 350 accelerated solvent extraction instruments.
Accelerated solvent extraction environmental applications summary. Ase 150350 5200 ml solvent usage are based on a per sample basis. Accelerated solvent extraction ase as a sample extraction. Dionex accelerated solvent extraction ase 150 and 350. Accelerated solvent extraction ase is an extraction technique that speeds the extraction process and reduces the total amount of solvent used. Ase abbreviation stands for accelerated solvent extraction. Determination of pesticides and persistent organic.
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